Oral Presentation Australian Society for Microbiology Annual Scientific Meeting 2023

Development towards a Chlamydia pecorum vaccine for koalas; Vaccine longevity, population effects and future directions (94059)

Samuel Phillips 1 , Jon Hanger 2 , Peter Timms 1
  1. Centre for Bioinnovation, University of the Sunshine coast, Sippy Downs, QLD, Australia
  2. Endeavour Veterinary Ecology, Toorbul, Queensland, Australia

The koala (Phascolarctos cinereus) is an endangered species under threat of extinction from several anthropogenic and natural risks, including disease related to infection with Chlamydia pecorum. For the past 15 years, Australian researchers have been developing a koala specific C. pecorum vaccine that has proven to be safe and effective for up to two years in wild koalas (Phillips et., al 2019). Previous trials were focused on a small region of Southeast Queensland where koala wildlife veterinarians were conducting a koala monitoring program. Five separate trials within this region have resulted in 165 of 680 koalas being vaccinated since 2013 (Khan et al., 2014, 2016a and b, Waugh etal., 2016 and Desclozeaux et al., 2017). For the past ten years clinical examinations have occurred at approximately 6 monthly intervals for all 680 koalas in this region. Analysis of both death in the presence of disease and the occurrence of disease in this large 10-year longitudinal study identified that vaccination significantly increased a vaccinated koalas protection from disease and death due to Chlamydial infection. This analysis identified that protection was not influenced by sex and that the vaccine showed a 65% reduction in disease mortality and a 46% reduction in signs of disease at a 24% vaccine coverage. Furthermore, vaccination was significantly associated with an increase in the koala’s survival.

These findings indicate that vaccination of wild koalas within Australia could result in increased protection against disease and a decreased mortality due to chlamydial disease. Furthermore, these findings also align with modelling data (Craig et al., 2014) that indicates at these levels of effectiveness, koala populations could be recovered from decline within 5 years of vaccinating 20% of a population.

  1. Khan, S.A., et al., Antibody and cytokine responses of koalas (Phascolarctos cinereus) vaccinated with Recombinant Chlamydial Major Outer Membrane Protein (MOMP) with two different adjuvants. PLoS ONE, 2016. 11(5).
  2. Khan, S.A., et al., Humoral immune responses in koalas (Phascolarctos cinereus) either naturally infected with Chlamydia pecorum or following administration of a recombinant chlamydial major outer membrane protein vaccine. Vaccine, 2016. 34(6): p. 775-782.
  3. Khan, S.A., et al., Vaccination of koalas (Phascolarctos cinereus) with a recombinant chlamydial major outer membrane protein adjuvanted with poly I: C, a host defense peptide and polyphosphazine, elicits strong and long lasting cellular and humoral immune responses. Vaccine, 2014. 32(44): p. 5781-5786.
  4. Waugh, C., et al., A prototype recombinant-protein based Chlamydia pecorum vaccine results in reduced chlamydial burden and less clinical disease in free-ranging koalas (Phascolarctos cinereus). PLoS ONE, 2016. 11(1).
  5. Desclozeaux, M., et al., Immunization of a wild koala population with a recombinant Chlamydia pecorum Major Outer Membrane Protein (MOMP) or Polymorphic Membrane Protein (PMP) based vaccine: New insights into immune response, protection and clearance. PLoS ONE, 2017. 12(6).
  6. Craig, A.P., et al., A 5-year Chlamydia vaccination programme could reverse disease-related koala population decline: Predictions from a mathematical model using field data. Vaccine, 2014. 32(33): p. 4163-4170.
  7. Phillips, S., B.L. Quigley, and P. Timms, Seventy years of Chlamydia vaccine research - Limitations of the past and directions for the future. Frontiers in Microbiology, 2019. 10(JAN).