In 2019, 4.95 million deaths were associated with antimicrobial resistance (AMR) worldwide. The human gastrointestinal microbiome has been identified as a source of acquired resistance in pathogens. Previously, culture-independent metagenomics was the primary method for identifying antimicrobial resistance in gastrointestinal microbiota. However, this approach cannot identify novel AMR genes or determine their expression or functionality within the cell. To overcome these limitations, our study utilised novel culturing on YCFA media in anaerobic conditions, which has been shown to allow for 96% of the gastrointestinal microbiota to be cultured. This method was used to culture 10 faecal samples from healthy individuals, with and without six common orally administered antimicrobials (Amoxicillin, Amoxicillin-Clavulanic acid, Cefalexin, Ciprofloxacin, Clindamycin and Doxycycline). The resultant 1058 colonies were picked, identified through 16S rRNA sequencing and resistance to each of the antimicrobials was confirmed in broth growth. Genes associated with resistance or sensitivity to the antimicrobials were identified through combining whole genome sequencing data and phenotypic (growth in broth) data of 98 isolates. There were 2050 genes associated with resistance to Amoxicillin-Clavulanic acid, 310 genes associated with resistance to Ciprofloxacin, 220 genes associated with resistance to Clindamycin, one gene associated with resistance to Doxycycline and no genes found to be associated with resistance to Amoxicillin or Cefalexin resistance. Of the genes associated with resistance, four are known antimicrobial resistance genes, whilst the remaining 2577 genes require further analysis to determine causation. Coupled with phenotypically validating this resistance, this will allow for identification of novel genetic elements responsible for phenotypic AMR within the microbiome, providing a greater understanding for how the microbiome may be spreading AMR.