This talk will give an overview of microbiology laboratory capacity building at Prof. Johannes Hospital in West Timor, Indonesia. This work was done through the Flinders Overseas Health Group (FOHG), a not-for-profit organisation based in Australia providing multi-disciplinary collaborative professional development for health professionals in Nusa Tengarra Timor(NTT) Province. We will outline our work to date, provide insights into the challenges in an under-resourced setting and touch on the disparities in access to healthcare. The experiences of up-skilling laboratory staff, improving infection control standards and helping the overall health outcomes for patients will be presented.