Routine laboratory investigation for diarrhoeal diseases received at NPV includes bacterial culture for Salmonella, Shigella and Campylobacter. Vibrio, Aeromonas and Plesiomonas. Extra media is added if specifically requested or if suggestive history is provided. Normally, results for the screening of these enteric pathogens are reported after 48 hours of culturing.
Clostridium difficile toxin (CDT) is currently tested on Genexpert upon request. Requests for bacterial, viral or parasite multiplex PCR are referred to external labs such as The Alfred Pathology Service, St. Vincent’s Pathology and Monash Pathology. Utilising Seegene Allplex GI Bacteria and Viral Assay, all non-parasitic faecal GI investigations can be consolidated into 2 panels and performed in-house, resulting in:
The following Allpex kits used with the verification study includes:
Allplex GI-Bacteria(I) Assay - multiplex real-time PCR system for the detection of Shigella spp./Enteroinvasive Escherichia coli (EIEC), Campylobacter spp., Yersinia enterocolitica, Vibrio spp., Clostridium difficile toxin B (CDT), Aeromonas spp. and Salmonella spp. in human stool specimens.
Allplex GI-Virus Assay - multiplex real-time one-step RT-PCR system for detection of Norovirus GI, Norovirus GII, Rotavirus A, Adenovirus F (Serotype 40/41), Astrovirus and Sapovirus (Genogroups G1, 2, 4, 5) in human stool specimens. Requests for culture, viral or CDT can be covered by the use of these 2 assays.