The MarR family of transcriptional regulators control adaption of bacteria to different environments. The MarR family protein MprA regulates expression of the EmrAB-TolC drug efflux pump, as well as capsule and flagella expression. While MprA provides an intriguing link between the coordinated expression of antibiotic resistance and virulence determinants, its regulon and mechanisms of transcriptional control have not been determined. Transcriptome analysis and genome-wide protein-DNA mapping were used to define the MprA regulon in uropathogenic E. coli (UPEC). Genes that were both directly regulated and differentially expressed were validated using promoter-reporter plasmids and electrophoretic mobility shift assays (EMSA). Two novel targets were validated at the protein and phenotypic levels using western blotting and growth kinetics. We identified 66 target genes of MprA, including 35 new genes, and show that 37 genes are directly regulated. Promoter-reporter plasmids validated that the promoters of these genes were responsible for differential expression, and EMSAs confirmed both direct and specific interaction of MprA with these promoters. The novel MprA-regulated targets included a gene encoding a common lipoprotein and genes involved in sialic acid catabolism, which were confirmed at the protein and phenotypic levels, respectively. We revealed a new role for MprA as a central activator of sialic acid scavenging, catabolism and utilisation in the production of a polysaccharide capsule. We have elucidated the regulon and protein-DNA interactions of MprA, a global regulator that coordinates the expression of both antibiotic resistance and virulence determinants in UPEC, a clinically important antibiotic resistant pathogen.